STUDIES ON HUMAN GROWTH HORMONE. I. A RADIO-IMMUNOASSAY FOR HUMAN GROWTH HORMONE*
نویسندگان
چکیده
منابع مشابه
Immunoassay for Human Growth Hormone
Since highly purified human pituitary growth hormone has become available, much information concerning its metabolic effects has been accumulated. However, simple and specific methods of measuring endogenous levels of human growth hormone have been difficult to achieve. Attempts to assay human growth hormone by injecting whole serum into hypophysectomized rats and measuring the width of the tib...
متن کاملStudies with human growth hormone.
Let me tell you at the outset of my appreciation of the great honour you have done me in asking me to deliver the first Lawson Wilkins Memorial Lecture. I accepted this invitation with eagerness because I cherish any link that binds Lawson Wilkins' life and work to mine. I often turn to the page in the second edition of his great textbook in which he proudly listed the 31 Associates and Fellows...
متن کاملA radio-immunoelectrophoretic assay for human growth hormone.
A number of attempts have been made to measure the amount of growth hormone in human plasma or in extracts thereof. By using the tibia-line biological assay Gemzell (1959) and Forsman & Gemzell (1959) detected growth hormone in extracts from subjects with acromegaly or diabetes but not in extracts of normal plasma. Owing to the lack of sensitivity and specificity of biological assays for growth...
متن کاملRT-PCR MEDIATED CLONING OF HUMAN GROWTH HORMONE GENE AND I TS EXPRESSION IN E. coli
Human growth hormone (hGH) genomic sequence containing 5 exons and 4 introns was cloned in pcDNA-3 and the constructed plasmid was subsequently used for transfection ofNlli-3T3 cell line using lipofection technique. Expression of hGH in stably transfected cells was assayed using ELISA. Total RNA was extracted from transfected cells and hGH cDNA was amplified by RT-PCR using specific primers...
متن کاملStudies with Human Growth Hormone * DOUGLAS HUBBLE
Let me tell you at the outset of my appreciation of the great honour you have done me in asking me to deliver the first Lawson Wilkins Memorial Lecture. I accepted this invitation with eagerness because I cherish any link that binds Lawson Wilkins' life and work to mine. I often turn to the page in the second edition of his great textbook in which he proudly listed the 31 Associates and Fellows...
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ژورنال
عنوان ژورنال: Journal of Clinical Investigation
سال: 1962
ISSN: 0021-9738
DOI: 10.1172/jci104478